How do enzymes like trypsin release cells from tissues to create preparations of single cells? The enzymes digest extracellular domains of proteins that mediate endocytosis. The enzymes digest extracellular domains of proteins that mediate cytoskeletal function. The enzymes digest extracellular domains of proteins that mediate cell adhesion. The enzymes digest intracellular domains of proteins that mediate cell adhesion. The enzymes digest intracellular domains of proteins that mediate cytoskeletal function.