To investigate a possible relationship between two factors, such as acidity and solubility, you must first identify the variables involved in the experiment. Identify the independent variable, the
dependent variable, and any possible confounding variables within this experiment.

answer: tributaries flow into rivers.

wetland is a biome that develops when the water gets accumulated in a land exhibiting a huge depression. these form either seasonally or permanently. the water in the wetland comes from rivers, lakes, ocean, flood, rain or other sources. the wetlands includes mangroves, marshes and peatland.

tributaries flow into the rivers develops a wetland because such water will get deposited in the depression and will restrict the original flow of tributary water. hence, water with low drainage current combines with the river water.

this protocol describes a detailed procedure for intracellular flow cytometry staining.