The chemiosmotic model requires an intact outer mitochondrial membrane. is supported by the observation that, when isolated mitochondria have an artificial electrochemical gradient (higher pH in the matrix) imposed on them, electrons spontaneously flow through the respiratory chain. is supported by the observation that isolated mitochondria that are actively respiring cause the pH of the solution they are in to increase. requires that mitochondrial ATP synthesis and electron flow through the respiratory chain be obligately coupled.

requires that mitochondrial ATP synthesis and electron flow through the respiratory chain be obligately coupled.

Explanation:

The chemosmotic model was a concept formulated by Peter Mitchell, who stated that during the phases of electron transport in the respiratory chain, an intense release of energy occurs. This released energy allows hydrogen ions to be transported from within the mitochondrial matrix to the intercellular medium. The result of all of this is the occurrence of a chemosmotic gradient. However, when interpreting what occurs in the chemosmotic model, we can see that this model requires that the mitochondrial synthesis of ATP and the flow of electrons through the respiratory chain must be coupled.

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