Choose the PCR primer pair that will amplify the breakpoint of a deletion of the segment of DNA between lines 1and 2. 5' 3'
3' 5'
a. 5- AGCTAAGGCCTTTCGA and 5'-CCACGGGTACCTATAA
b. 5'- TCGATTCCGGAAAGCT and 5'-ACGTCCCGGGAAACTA
c. 5- TCGATTCCGGAAAGCT and 5'-CCACGGGTACCTATAA
d. 5'- TCGATTCCGGAAAGCT and 5'- GGTGCCCATGGATATT
e. 5' - GGTGCCCATGGATATT and 5' ACCTAGGTAAGCGCCG

a. 5- AGCTAAGGCCTTTCGA and 5'-CCACGGGTACCTATAA

Explanation:

The Polymerase Chain Reaction (PCR) primers are short nucleotide sequences (composed of approximately 20  nucleotides in size) flanking a target sequence that is amplified during PCR reaction. These primers bind to the DNA template by means of complementary base pairing in order to make billions of copies of a target DNA region, which is then visualized as a band by electrophoresis. In this case, PCR primers from the item a- (i.e., AGCTAAGGCCTTTCGA and CCACGGGTACCTATAA) will bind to the DNA template of lines 1 and 2 in order to amplify a continuous region:

Schematically:

The Foward primer AGCTAAGGCCTTTCGA binds by complementary base pairing:

5'_(TCGATTCCGGAAAGCT)_3'

The Reverse primer CCACGGGTACCTATAA binds by reverse complementary base pairing:

3'_(TTATAGGTACCCGTGG)_5'

burning of wood and coal

explanation:

the panel also concluded there's a better than 95 percent probability that human-produced greenhouse gases such as carbon dioxide, methane and nitrous oxide have caused much of the observed increase in earth's temperatures over the past 50 years.

4.) metal

"-the movement of carbon from the nonliving environment into living things and back to the environment

-carbon is the essential component of proteins, fats, and carbohydrates (which make up all organisms)

-carbon exists in air, water, and living organisms

-producers convert carbon dioxide in the atmosphere into carbohydrates during photosynthesis

-bacteria in the atmosphere cycles through the system in filtration units

-in cellular respiration, carbon is released back into the atmosphere as carbon dioxide

-some carbon is stored in limestone (forming one of the largest "carbon sinks" on earth)

-largest carbon reservoir on earth= limestone

-carbon stored in the bodies of organisms as fat, oils, or other molecules may be released into the soil or air when the organism dies

-this may form deposits of coal, oil, or natural gas (fossil fuels, which increased the atmospheric carbon dioxide)

-fossil fuels store carbon left over from bodies of organisms that died millions of years ago

-they are located in the surface of earth, deep within the earth and beneath the ocean floor."