A researcher wants to introduce the human gene encoding tissue plasminogen activator (used to dissolve blood clots) into a mammal so that the protein will be secreted into the milk of the mammary gland. For the researcher to succeed, the gene should be:

In the given case, for the researcher to succeed, there is a need to place the gene next to the promoter sequence, which gets expressed in the mammary cells. The sequences of DNA, which illustrate that where the transcription of a gene by the RNA polymerase initiates is known as the promoter. These sequences are generally situated at the 5 prime terminals of the initiation site of transcription or are situated directly upstream.

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