21. When looking at the effect of pH on sucrase activity, what was the wavelength of light used in the spectrophotometer?

540nm

Explanation:

Sucrase is one of the digestive enzymes responsible for the digestion of sucrose containing foods. Its major function is the catalysis of the hydrolysis of sucrose into its subunits; fructose and glucose.

Enzymes, as biochemical catalysts always function maximally at an optimum pH. As the pH of the system departs from the ideal pH for any enzyme, the activity of that enzyme decreases accordingly.

The activity of sucrose is studied using a spectrophotometer of wavelength about 540nm. As the pH of the solution departs from the specific ideal pH of sucrase, its activity decreases as stated above.