You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during the absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during the absorptive state. provide answers to the following questions?1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide genefrom the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 whichhas a 5-recognition site and a 3- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?

Schools are being forced to embrace computers. unlike their "digital immigrant" teachers, these students are "digital natives", born into a world where technology is an integral part of everyday life. almost every student has access to a multitude of computers, laptops and tablet devices. it would be crazy to assume an assignment would  notbe written on computer in this day and age.many schools now have a 1-to-1 computer policy, and many districts and states in the united states have mandated computers or ipads across the state for every student,  including los angeles, the second largest school district in the country.but the thing is, when they tried it in la, it wasn't the success they hoped for. that's because the tablets weren't used as much as they expected. rather, it appeared there was a gap between the digital native's love of technology and their use of it in the classroom.this conflicted relationship with computers is also underscored by much of the literature on education. it is also highlighted in professional development and conferences such as  edutech.

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Ithink most frequently occurring lab-acquired infection. proper way to clean up a small blood spill that has dried on surface are to be used when caring for all patients at all times. which of the following actions would violate a lab safety rule.