A single point mutation in a gene results in a nonfunctional protein. Individuals heterozygous for this mutation were identified using a Southern blot. Which pair of wild-type (WT) and mutant alleles most likely contains the mutation? A. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCGATGCTTAGGCATCT-3
B. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCGAAGCTTAGGCATAT-3
C. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTTGAAGCTTAGGCATCT-3
D. WT 5-TAGTCGAAGCTTAGGCATCT-3
Mutant 5-TAGTCCAAGCTTAGGCATCT-3

C. WT 5-TAGTCGAAGCTTAGGCATCT-3

Mutant 5-TAGTTGAAGCTTAGGCATCT-3

Explanation:

In the wild type, the DNA sequence encodes the following aminoacidic segment: SRSLGI; while the mutant form has a stop codon in the second codon (TGA). A stop codon is a trinucleotide sequence in the messenger RNA (mRNA) that indicates a stop signal in protein synthesis. There are three types of stop codons: UGA, UAG and UAA, where U represents Uracile in the mRNA instead of Thymine in the DNA sequence.

Representation:  

C. WT 5-T AGT CGA AGC TTA GGC ATC T-3 >> 5'3' Frame 1 = SRSLGI

Mutant 5-T AGT TGA AGC TTA GGC ATC T-3 >> 5'3' Frame 1 = S-, where - represents a stop codon (TGA) and therefore it is the end of the protein.

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the oceanic zone is typically defined as the area of the ocean lying beyond the continental shelf, but operationally is often referred to as beginning where the water depths drop to below 100 meters (328 feet), seaward from the coast to the open ocean.

explanation: