Biology, 11.06.2020 21:57 james22000

Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a DNA sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (PCR). The amplified DNA is then spotted to filters and hybridized with probes that recognize single nucleotide differences. Four primers (called A, B, D, and E) were used to amplify DNA samples and ASO probes were used to determine the allele present (either allele 1 or 2). "X" indicates the allele is present and "–" indicates the allele is not present. What are genotypes of this male's parents for A, B, D, E loci?

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