Most mammalian cell types do not undergo homologous recombination (HR), and even mouse ES cells do so at low frequency - about 1/200000 cells. However, if you first introduce a crRNA designed to target a mouse genomic sequence next to a PAM site, plus a source of Cas9 protein and a plasmid with homology arms, most mouse cell types will undergo HR with very high frequency -as high as 1/5 or more of the treated cells. What does this tell you about the mechanism of HR in mammalian cells

What is the first step in a life cycle of a plant reproduce sexually

Write a paragraph at least 5 sentences “why all vaccinations should be mandatory”

Mouse liver cells were homogenized and the homogenate subjected to equilibrium density-gradient centrifugation with sucrose gradients. fractions obtained from these gradients were assayed for marker molecules (i.e., molecules that are limited to specific organelles). the results of these assays are shown in the figure. the marker molecules have the following functions: cytochrome oxidase is an enzyme involved in the process by which atp is formed in the complete aerobic degradation of glucose or fatty acids; ribosomal rna forms part of the protein-synthesizing ribosomes; catalase catalyzes decomposition of hydrogen peroxide; acid phosphatase hydrolysis monophosphoric esters at acid ph; cytidylyltransferase is involved in phospholipid biosynthesis; and amino acid permease aids in transport of amino acids across membranes. a) name the marker molecule and give the number of the fraction that is most enriched for each of the following cell components: lysosomes; peroxisomes; mitochondria; plasma membrane; rough endoplasmic reticulum; smooth endoplasmic reticulum.