subject
Biology, 05.05.2020 01:45 jamarstand

You have identified a novel gene, Ums1, whose protein product is a component of the RISC in the nematode C. elegans. You believe this protein (like all the RISC proteins) is critical for the production of miRNAs, in particular ones that slice the mRNA for a gene called Trtn1. Describe how you would use RNAi in worm lysates to knockdown Ums1 and show that slicing of Trtn1 is inhibited without Ums1. Include details like what part of Ums1 you would target with siRNAs, how trigger RNA would be delivered to the worms, and how you assay for sliced or unsliced Trtn1 mRNA. Show what positive results would look like (probably with an illustration of an autorad or bar graphs).

ansver
Answers: 3

Another question on Biology

question
Biology, 21.06.2019 21:20
Which codon is the code for the amino acid histidine (his)?
Answers: 1
question
Biology, 22.06.2019 02:30
The theories on the expansion of galaxies maybe re-examined to strengthen science should not be tested to strengthen science will weaken scientific knowledge if they change will become laws if they don't change
Answers: 1
question
Biology, 22.06.2019 03:00
Which of the following is the source of energy for photosynthesis? a.) glucose b.) chlorophyll c.) carbon dioxide d.) sunlight
Answers: 1
question
Biology, 22.06.2019 04:00
1) strawberry plants typically reproduce by making runners, which are miniature versions of themselves, that grow off of the roots and stems of the parent. this type of vegetative reproduction is known as a) pollination. b) fragmentation. c) binary fission. d) vegetative propogation.
Answers: 2
You know the right answer?
You have identified a novel gene, Ums1, whose protein product is a component of the RISC in the nema...
Questions
question
Mathematics, 10.03.2021 19:10
Questions on the website: 13722367