Why must an in vitro observation be confirmed in vivo?
A. A living thing does not make its own enzymes.
B. A test tube is exactly like a living cell.
O
c. Some enzymes behave unnaturally in a test tube.
O
D. Observations in a test tube are easy to make.

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.