Each of the proteins involved in DNA replication plays an important role. If one of these proteins was not functional it would have significant consequences. For each of the proteins involved in DNA replication, imagine it was not functional, then match it to the corresponding consequence that would result. 1st choice No new DNA nucleotides would be added to the growing strand. 2nd choice The two parental strands of DNA would come back together before new daughter strands could be made. 3rd choice The double-stranded DNA would not unwind. 4th choice No RNA-primer would be made. 5th choice Gaps between lagging strand segments would never be closed. 6th choice Double-stranded DNA would become tangled, preventing replication from proceeding.

Explanation:

1. Topoisomerase is an enzyme helps in over winding Or underwinding of DNA during replication. Helps in the topological correction. So non-functional topoisomerase leads to tangled DNA and prevents replication..

2. Primases are the enzymes helps in the synthesis of short RNA sequences used as primers in replication. Non functional primase leads to no primer synthesis.  

3. DNA Polymerase is an enzyme helps in the addition of new nucleotide to the growing strand in replication. Non functional DNA polymerase prevents the strand growing as no new nucleotides were added.  

4. Helicases are the enzymes helps in the separation of double strands into single and helps in each strand to be copied. Non functional helicase leads to prevention of unwinding of strands and replication inhibition.  

5. ligase is an enzyme that joins nicks(small gaps) in the DNA strand by creating ester bond. Non functional ligase leads to unjointed gaps of lagging strand.  

6. Single strand binding proteins(SSB's) are the small proteins that binds to the single strands of DNA and holds them in a place not to get together while replicating. Non functional SSB's leads to parental strands come back together and prevents replication.

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