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The discovery, characterization, and the application of the CRISPR/CAS system has revolutionized the study of genetics and genetic engineering. Please select all correct answers regarding its mechanism of action and application. Multiple correct answers are possible, and partial credit will be given.
A. CRISPR sequences in bacteria resemble those from bacteriophage and plamid DNA.
B. For reverse genetics studies, CRISPR/CAS can be used to replace wildtype alleles with mutant alleles which can knockout the function of the target genes.
C. CRISPR is a DNA sequence in the bacterial chromosome.
D. The guide RNA directs CAS to specific DNA sequences.
E. The CRISPR/CAS system was discovered in bacteria, and its natural function is the equivalent of an immune system in bacteria for "remembering" viral infections and for attacking viral DNA in future infections.
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