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Biology, 16.04.2020 20:16 hailenardos5

You are working on a protein containing no tyrosine residues and 2 tryptophan residues. Excitation at 295 nm yields the observation of an emissions spectrum with distinct peaks at 315 and 350 nm. Using your knowledge of tryptophan emissions behavior please interpret these data. Specific questions to address include: 1) Why would you expect to observe two distinct peaks? 2) What can you say about the microenvironment for each tryptophan residue? 3) What can be inferred about the possible electronic transitions available to these tryptophan resides? 4) For the alternative scenario where this protein contains 1 tyrosine residue, would these data collected under identical conditions be interpreted differently?

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