The following steps are all involved in genetic recombination:

1. Screening for cells that contain the recombined gene.
2. Cutting the vector with restriction enzyme.
3. Mixing the gene of interest with the vector.
4. Isolating the gene of interest from its original source.
5. Ligating the gene of interest and the vector together.

The following sequence of these five steps would be typical:
O 1) 1, 2, 3, 4, 5
O2) 2, 3, 5, 1,4
O3) 5, 4, 3, 2, 1
4) 4, 2, 3, 5, 1
5) 2, 3, 5, 4, 1

4) 4, 2, 3, 5, 1

Explanation:

Gene recombination technology involves the combination of DNA from two different species. A gene of interest is inserted into a vector to produce a recombinant DNA.

The process of DNA recombination technology involves a series of steps:

The first step involves the isolation of gene of interest from its source.The DNA of the vector organism (usually, the plasmid of a bacterium) is then cut using a restriction enzyme.The isolated gene of interest is then mixed with the DNA of the vector organism. This leads to pairing of the DNA bases at the sticky ends of the genes.The two genes are then joined together by an enzyme known as DNA ligase.The recombined DNA is then inserted into the host organism. Multiple host organism are used and the ones in which the gene of interest has been successfully integrated into their genome are screened out using an appropriate method.

The steps can be re-arranged thus:

4. Isolating the gene of interest from its original source.

2. Cutting the vector with restriction enzyme.

3. Mixing the gene of interest with the vector.

5. Ligating the gene of interest and the vector together.

1. Screening for cells that contain the recombined gene.

The correct option is option 4.

Might need you in a few weeks c:

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