Describe the role of restriction enzymes and DNA ligase in the process of making recombinant plasmids during the process of plasmid DNA transformation.

In many cases, cloning involves inserting the gene into a piece of circular DNA called a plasmid, which can be copied in bacteria. ... If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.

b. the carbon cycle ensures living things have a source of hydrogen