PNGase F cleaves N-linked oligosaccharides and removes the entire glycan from the polypeptide chain by hydrolyzing the amine on the amino acid sidechain that connects the glycan to the polypeptide chain. Given the following polypeptide sequence that was treated with PNGase F, where are the site(s) for glycosylation (pick the appropriate Section)? HINT: Where does N-linked glycosylation occur and what would happen to the glycosylated residue when PNGase F hydrolyzes the bond between glycan and amino acid?
AEWQNITKGF SEDRLLIAGT DSSDLQQILS LLESNKDLLL

Section 1 Section2 Section 3 Section 4

a. Section 1
b. Section 2
c. Section 3
d. Section4

sodium i think the chemical element of atomic number 11, a soft silver-white reactive metal of the alkali metal group.

picture a world where computers are building made-to-order personalized robotic arms and synthetic organs, drones are circulating above mars! due to the rapid growth of technology, the future has never been more unpredictable, and it presents a huge challenge in education: we are teaching students how to work in a future that we have never been unsure of. it is widely predicted that in eight years’ time, there will be more jobs in the fields of science, mathematics, technology and engineering than in any other discipline. and, as the technology continues to develop, it will soon become clear that addressing social problems will involve more innovative thinking, and analytical and technical knowledge than ever before. undeniably, there are no subjects that encourage innovative and creative thinking, deep thought, problem solving and independence more than the stem subjects. while it is widely commented on that there is a need to reimagine the ways stem subjects are taught in schools by embracing a more interactive and innovative approach to appeal to more students, it should come as no surprise that some of the most reputable educational organizations in the world are already implementing changes to embrace stem. harvard, for example, announced in 2014 that they were increasing their computer science faculty by over 50% and has since introduced computer science as a core subject for all freshmen. the urgency of supporting stem subjects at a young age is becoming more relevant than ever, as adopting a keen interest in the curricula needs to happen early. however, beyond the age issue is one of gender.

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