The lowest trophic level in an 1) has the lowest quantity of stored energy 2) has the greatest biomass 3) is made up of heterotrophs 4) contains detritovores

In describing the energy transfer of trophic levels, energy is lost as we move up from a lower level to a higher level. This is because energy is used when for example a primary consumer does body processes before it is eaten by another consumer.  hence the lowest trophic level belongs to those which 2) has the greatest biomass