Which of the statements regarding DNA replication are true? Replication takes place in the 5 ′ 5′ to 3 ′ 3′ direction on the leading strand and in the 3 ′ 3′ to 5 ′ 5′ direction on the lagging strand. Replication usually takes place in the 5 ′ 5′ to 3 ′ 3′ direction. Eukaryotic chromosomes have more than one origin of replication. Leading strand fragments (Okazaki fragments) are synthesized semi‑discontinuously and must be joined by DNA ligase. New nucleotides add to the 5 ′ 5′ phosphate of the growing DNA molecule.