Identify the statements that are features of a promoter. In prokaryotes, the promoter contains a –35 and –10 region upstream of the transcription start site. In eukaryotes, the promoter is recognized by a sigma factor subunit, which must bind to the promoter before forming the preinitiation complex. In both prokaryotes and eukaryotes, the promoter is located in the 5 ′ direction, upstream from the transcription start site. In eukaryotes, the promoter often contains a TATA box, which is where the TATA‑binding protein binds. In eukaryotes, the promoter attracts the small and large ribosomal subunits with the help of initiation factors.