A cloning vector was digested with only one restriction enzyme. Immediately after a single ORF flanked with overhanging ends, generated with the same enzyme, was cloned into the plasmid. The ORF was inserted downstream a single inducible promoter. Which of the following is the correct cloning rate expected?Note: Only plasmids carrying inserts should be considered. a. 25%b. 10%c.100%d. 50%

Which organelles are labeled d, and what is one feature that distinguishes them from the other labeled organelles chloroplasts the only organelles that produce sugars from sunlight mosomes, only found in animal and bacterial cells centricles only found in animal cells mitochondra, the only energo-generating structures found in cells

Clues to ecological principles are given in numerous passages of the bible. a bible study of ecology actually opens the doors to better understanding of god's love and concern for the earth. use a concordance to locate bible passages associated with words concerning our stewardship of the earth. you may use a dictionary to find synonyms for these words: dominion replenish subdue judgment stewardship find each of the passages using these words related to principles of ecology. list all passages by quote, book, chapter, and verse under each word. use your own interpretation to rewrite what you think each verse is saying. after your discussion of each of these words, ask one other person to explain his understanding of the word and a related verse without any hints. summarize what he tells you. a concluding paragraph for each of the words should be written noting similarities and differences between your interpretation and that of each person consulted.

For the strategies below, decide whether they are best described as demand-side solutions or supply-side solutions to meeting human needs for fresh water. remember that a demand-side solution reduces demand for fresh water, whereas a supply-side solution increases the supply of fresh water. (a) damming a river to create a reservoir (b) desalinating seawater (c) a nation invading a neighboring country to access their rivers (d) raising water prices for consumers and businesses (e) providing tax breaks for companies that use less water (f) replacing inefficient irrigation with more-efficient drip irrigation to grow crops (g) requiring low-flow showerheads in all new homes