The cell-free in vitro translation system utilized by Nirenberg and Matthaei to determine the first "words" in the genetic code utilized addition of into purified E. coli extracts:

Radio active amino acids

Explanation:

The Nirenberg and Matthaei performed the experiment which showed the relationship between the nitrogenous bases present on the mRNA and the amino acids.

They knew that the amino acids are coded by the bases on the mRNA and they cultured the E.coli bacteria, ruptured them and released the cytoplasmic content from them.

Then they added the artificially synthesized RNA molecule attached with radioactive molecules and added them to different tubes. From this, they concluded that there is 64 combination of triplets which are responsible for the production of a variety of amino acids.

Thus, Radioactive amino acids are the correct answer.

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these macromolecules could either be amino acids, proteins or nucleic acids. they are responsible for the chemical changes and reactions that affects largely the cell and its composition. take for instance the cytoplasm of the cell where these organelles are settled. these simple organelles are composed of macromolecules which ignites and catalyses different functions that enables cells, in macro-perspective in motion and metabolism. in intestines for example, metabolism happens and breaking down parts of a food to simpler compounds that are used and these nutrients delivered throughout the body and again broken down by into smaller components.