In which calluses did the new gene insert inside an existing corn gene?

You can check your ligation products by gel electrophoresis or PCR using plasmid primers across the insert but the number of ligation products and their low concentration makes analysis by agarose gel electrophoresis an impractical method. So sequencing could be a better option.

Explanation:

i believe it would be the lungs.

enzyme active site and substrate specificity

enzymes bind with chemical reactants called substrates. there may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. in some reactions, a single-reactant substrate is broken down into multiple products