You have now added both PCR primers and successfully run the PCR, but you notice that the quantity of product generated is low. The graduate student you work with mentions that your expected product is quite long and that you may want to alter your PCR conditions to increase efficiency of product generation. Which of the following would help increase the amount of long PCR product generated? Choose one: A. increasing the annealing temperature when primers hybridize to the template DNA B. increasing the elongation temperature when DNA polymerase synthesizes the new DNA C. increasing the elongation time when DNA polymerase synthesizes the new DNA D. increasing the annealing time when primers hybridize to the template DNA

a

explanation:

interphase

the period of cell growth when the cell synthesizes new molecules and organelles. the cell is not dividing and it constitutes the majority of the time spent in the cell cycle

prophase

changes occur in the nucleus and cytoplasm. the chromatin condenses to form structures (sister chromatids) visible with a light microscope and the mitotic spindle begins to form, but the nucleus is still in tact

prometaphase

the nuclear envelope fragments and the spindle microtubules attach to the kinetochores of the sister chromatids

metaphase

all the cell's duplicated chromosomes are lined up at an imaginary plane equidistant between the poles of the mitotic spindle

anaphase

begins when sister chromatids separate from each other and ending when a complete set of daughter chromosomes arrives at each of the two poles of the cell

telophase

the daughter nuclei form at the two poles of a cell

cytokinesis

the division of a cytoplasm to form two separate daughter cells. usually occurs during telophase during mitosis. this and mitosis make up to mitotic (m) phase of the cell cycle