There are many well-known mutant forms of Hb. These forms are usually named after the region where they are first identified. Hb 'Philly' is a form of hemoglobin that is destabilized by having an F substitute for a Y in the alpha-subunits. The Y normally participates in the hydrogen-bonded network that helps to knit together the alpha1-beta1 interface. Would we say that this mutation destabilizes 1', 2', 3' or 4' structure? Pick one (the best answer) and explain why F causes this problem.