The A260/A280 ratio method estimates protein by measuring the absorbance maximum at 280nm caused by the amino acids, , and . Since also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at and using the following equation:

Explanation:

The A260/A280 ratio method estimates protein purity by measuring the absorbance maximum at 280nm caused by the amino acids aromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure. Since nucleic acid also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at 260nm and using the following equation.

answer; ///i believe the answer your looking for is (ribosomes);

substitution is the answer in this case