Which one of the following activities would LEAST likely be found in a topoisomerase?

(A)cutting a phosphodiester linkage
(B)inducing or relaxing twists in double-stranded DNA
(C)Topoisomerases are equally likely to perform all of these functions.
(D)adding nucleotides to the end of a DNA strand
(E)reforming phosphodiester linkages

Explanation:

Topoisomerases are enzymes that participate in the or underwinding / inducing or relaxing twists in double-stranded DNA. During DNA replication or trancription ,the twist are generated ahead of the replication fork so the topo-isomerases removes these twists. In case of bacteria, topoisomerse is known as gyrase.

Topoisomerases are very important in DNA replication and transcription.