Biology, 06.03.2020 03:46 Nbeans4962
Gram staining It is a differential staining method to differentiate between gram positive and gram-negative bacteria based on the differences in their cell wall composition. Gram positive bacteria has a thick meshwork of peptidoglycan layer due to which crystal violet iodine complexes get entrapped in between them and it appears purple. Gram negative bacteria does not have a thick meshwork of peptidoglycan, instead it is thin and therefore unable to trap crystal violet iodine complexes and loses purple color on alcohol wash. Rather, it gets a reddish oranges color on staining with safranine. Procedure - 1. On a slide, make bacterial smear. 2. Heat fix the cells. 3. Add a drop of crystal violet on the slide and wait for 1 minute. Wash off excess stain with water. 4. Add a drop of iodine on the slide and wait for 1 minute. Wash off the excess stain with water. 5. Wash with alcohol and wait for 30 seconds. Wash with water. 6. Wash with safranine and wait for 30 seconds. Wash with water. Gram positive will appear purple and gram negative will appear orange. Flu is causes by virus and therefore, cannot be detected by gram staining. Spore stain is primary staining method which uses malachite green dye to stain endospores. Simple stain is used to visualize bacteria by using only one stain. If it stains the bacteria, then it is positive stain and if it stains the background and bacteria is colorless then it is negative stain
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Gram staining It is a differential staining method to differentiate between gram positive and gram-n...
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