In one experiment, scientists raised mice in germ-free conditions so the mice lacked intestinal microbes. The mice were fed a low-fat diet rich in complex plant polysaccharides, such as cellulose, that are often called fiber.
When the mice were 12 weeks old, the scientists transplanted the microbial community from the intestine of a single "donor" mouse into all of the germ-free mice. Then they divided the mice randomly into two groups and fed each group a different diet.
Group 1 (the control group) continued to eat a low-fat, high-fiber diet.
Group 2 (the experimental group) ate a high-fat, high-sugar diet.
Identify the components of this experiment by matching the terms with the appropriate category below:
Factors being tested Factors controlled Factors to be measured
(independent variable) (Kept consistent) (dependent variable)
1. initial composition of the microbial community
2. age of the mice
3. change in body fat
4. diet
5. weight gain
6. final composition of the microbial community
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