When extrapolated from a standard curve, the Bradford data indicates the amount (in ) of protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein of the sample. The coomassie blue dye in the Bradford assay specifically binds primary and groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the the blue color will be, and the absorbance at 595nm will be. A sample with an unusually number of [give a specific example] amino acids will underestimate the total amount of protein present in the sample.