Thankfully, another lab has sequenced the bacterial genome, so you know where on the bacterial chromosome the genes you are interested in are located, as well as the DNA sequences before and after them. To remove the genes from the chromosome, you will use enzymes called restriction endonucleases that will cut the DNA strand at specific base sequences. This "digestion" will allow you to tailor the DNA fragment that you will be removing from the chromosome so that you may know where the cuts will be made and what additional DNA bases may come with the fragment containing the genes you are interested in.