In 1964, Nirenberg and Leder used the triplet binding assay to determine specific codon assignments. A complex of which of the following components was trapped in the nitrocellulose filter?

Charged tRNA

Explanation:

The first time the expereiment was performed was on the 15th of May in 1961 by two men  Marshall W. Nirenberg and his post doc, J. Heinrich Matthaei. This experiment is now called the Nirenberg and Matthaei experiment where they used ruptured E.coli cells, RNA and labelled tRNA to decipher the triplet codon that matched to specific amino acid. By the time the experiment was complete the men had found 64 amino acid codons which was a large contribution to genetics and molecular biology.

not too sure but maybe in the hot springs.

you didn't give any options but heres what i can tell you: an inference is likely to change based on additional observations. the latter is something that you have seen or sensed using your sensory organs while the former is like a conclusion out from what you have observed.