Biology, 27.02.2020 22:56 jsdhhddb8445
Like all enzymes, restriction endonucleases (REs) need to be handled very carefully to ensure that they do not denature. Which of the following is NOT recommended to protect these fragile structures from denaturation? a. REs should be stored in glycerol solutions, which do not freeze at temperatures below the freezing point of water.
b. Investigators should minimize the number of air bubbles introduced into RE solutions during pipetting.
c. REs should be stored at low temperatures, since the rate of denaturation increases with temperature.
d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
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