Biology, 25.02.2020 18:26 krutikov686

[methyl-14C]pyruvate was administered to isolated guinea pig liver cells in the presence of sufficient malonate to completely block the action of succinate dehydrogenase. After a time, isocitrate is isolated and found to contain radiolabeled carbon at both the —CH2— and the —CHOH— carbons, instead of just at

the —CH2— carbon. Explain how the —CHOH— got radiolabeled.

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