You have a scope whose lense has NA of 0.65. Your illumination source lambda is 550 nm (ie white light). Will you be able to see a bacterium 0.2 micron in diameter?

Well, both latitude and intensity of light affect temperature because, the higher the light intensity, the higher the temperature and since latitude is the measure of how close you are to the equator, then the closer to the equator you are, the higher the temperature is.