Biology, 13.02.2020 23:32 710jonathan
A plasmid was cleaved with several restriction enzymes, individually and in various combinations. The following fragment sizes (base pairs) were determined by agarose gel electrophoresis.
EcoRI 4364
AvaI 4364
PvuII 4364
PstI 4364
EcoRI AvaI 2938, 1426
EcoRI PstI 3608, 756
AvaI PvuII 3722, 642
AvaI PstI 2182 PvuII
PstI 2824, 1540 EcoRI
PvuII 2 296, 2068
Make a restriction map based on this data. Indicate the distances between sites. Why is only one band detected in the Ava I-PstI co-digest
Answers: 3
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A plasmid was cleaved with several restriction enzymes, individually and in various combinations. Th...
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