Put the following events in the construction of a recombinant DNA molecule in the correct order: a. ligation of desired gene to plasmid DNA b. introduction of the plasmid into bacteria c. restriction enzymes cut gene of interest and plasmid DNA d. growth of cells on an antibiotic-containing medium

Turtle hatchling survivorship rate is low in many turtle species due to predation. amanda researched the predatory rate on a species of turtle eggs in the red river. the eggs were harvested from trapped turtles and the egg's cloaca film (reproductive discharge) was either washed off or left on once gathered. research suggests that predators use the female's cloaca scent to locate the eggs. the eggs were only handled when wearing gloves and then reburied along islands where the turtles were trapped. the nests were monitored by cameras and manually on foot and data on nest predation were recorded. a. based on the experimental design, what is the dependent variable? b. based on the experimental design, what is the independent variable? c. based on the experimental design, what is the control?

Which would happen if more forests were cut down? a. the nitrogen cycle would be stopped b. nitrogen would be fixed by bacteria more quickly c. the amount of co2 in the atmosphere would increase d. fossil fuels would build up in the soil c the amount of co2 in the atmosphere would increase

1. watermelon, papaya, oranges, bananas, and lemons are included in this food group. vitamin c 2. you need 6 or more servings of this food group each day vitamins d and a 3. found in fruit and keeps blood vessels healthy calcium 4. vitamins found in milk bread and grain 5. a mineral that strengthens bones fruit