The protein pictured is bovine insulin. Determine the residue number and the size of the fragments that would be generated upon treatment with following proteases (1) trypsin, (2), chymotrypsin and (3) BrCN; (a) without DTT and (b) with DTT. (Where DTT breaks disulfide bonds).

The lake of the ozarks is a human-made lake, so it collects runoff from coal strip-mining, fertilizers, resort wastewaters, and septic drainages. the average lake temperature is between 10∘c and 21∘c. consider the physical requirements for growth and multiplication that would allow fecal coliforms to “blossom” in the lake of the ozarks. which of the following would accurately describe these organisms? check all that apply. psychrophiles facultative halophiles extremophiles mesophiles hyperthermophiles