Restriction enzymes, recombinant dna, and gene cloninga. which enzyme(s) will produce a dna fragment that contains the entire vgp gene (shown in red) and has "sticky ends"? b. the questions below will determine which restriction enzyme should be used to make a plasmid that contains both an intact vgp gene and a functional ampr gene. c. to sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampicillin and in a medium without ampicillin. demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow. d. to accomplish the screening, researchers synthesize a single-stranded dna probe using vgp mrna as a template. what will be the sequence of nucleotides in the probe?

Consider the motion of the balloon and its air contents in terms of momentum. in step 1 above, the total momentum of the balloon and its contents was zero. recall that momentum = mv. both the balloon and the air inside it had a velocity of zero and therefore the total momentum was zero. now think about what happened when the air escaped from the balloon. a certain mass of air accelerated in one direction. in order to keep the total momentum of the system zero, the balloon itself (which has mass) had to accelerate in the opposite direction. use this scenario to you explain why the soda can rotates when the water squirts out of the escape holes. what was your hypothesis concerning the water-filled can? according to your data, do you think your hypothesis was correct? (be sure to refer to your data when answering this question.) summarize any difficulties or problems you had in performing the experiment that might have affected the results. describe how you might change the procedure to avoid these problems. give at least one more example of newton's third law in everyday life.