2.4.2 quiz: mutations
question 2 of 10
2 points
the first step in the hybridization of radioactive dna and normal dna is to:
o
a. make a solution with the two types of dna.
o
b. cool a hot mixture of the two types of dna.
o
c. expose two types of dna to radiation.
d. heat a mixture of the two types of dna.

The first step that is involved in the hybridization of radioactive DNA and normal DNA is to heat a mixture of the two types of DNA.

Option D

Explanation:

Hybridization of radioactive DNA and normal DNA is a molecular biology technique that is used to analyse the similarities between the radioactive DNA and normal DNA for scientific purpose.

The first step involved in this hybridization process is heating of the two DNA at higher temperature and this heating process is known as DNA melting. This is usually preferred as a method to determine the genetic distance between two organisms.

The first step in the hybridization of radioactive DNA and normal DNA is to heat a mixture of the two types of DNA.

Explanation:

In the molecular probe technique, the probe sequence which is a single strand is radiolabelled and used to identify the complementary strand.

The temperature and ion concentration at which hybridisation is performed play a key role in the results obtained.

The hybridization results are directly related to Tm (melting temperature of DNA) of DNA. The first step of hybridisation requires 65 degrees (annealing temperature) so that 83 per cent of homologous pairs get bonded.