What are the similarities and differences between "template based" and "template free" methods for protein structure prediction?

In fact, the key difference between "template based" and "template free" structural prediction methods lies in the different conformational initialization methods. Template based prediction method obtains the initial conformation by searching and recognizing the known structure which has homology or structural similarity with the target protein as the template; template free prediction method usually constructs the initial conformation from the beginning with a small structure fragment.

At present, no matter which method is used, the known structural information in PDB database will be used to construct an initial conformation which is far better than random conformation, thus significantly reducing the pressure of conformation search, including reducing the amount of calculation and reducing the dependence on high-precision force field

Explanation:

http://www.creative-biostructure.com/protein-crystallization-and-structure-determination_13.htm

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

when you breathe in, or inhale, your diaphragm contracts (tightens) and moves downward. this increases the space in your chest cavity, into which your lungs expand. the intercostal muscles between your ribs also enlarge the chest cavity. they contract to pull your rib cage both upward and outward when you inhale.