Restriction enzymes, recombinant dna, and gene cloning
a. which enzyme(s) will produce a dna fragment that contains the entire vgp gene (shown in red) and has "sticky ends"?
b. the questions below will determine which restriction enzyme should be used to make a plasmid that contains both an intact vgp gene and a functional ampr gene.
c. to sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampicillin and in a medium without ampicillin. demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow.
d. to accomplish the screening, researchers synthesize a single-stranded dna probe using vgp mrna as a template. what will be the sequence of nucleotides in the probe?