Put the steps to produce spider silk protein in yeast in the correct order.

1. purify protein.
2. join yeast regulatory sequence and spider silk coding sequence by using enzymes. 3. insert recombinant gene into yeast cells.
4. use enzymes to remove yeast coding sequence.
5. allow yeast to express spider silk gene.

He did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis

Has regulatory areas for thirst, hunger, body temperature, water balance, and blood pressure

Does solar energy ever run out? brainliest