Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor viii. not all steps will be placed. isolate the human gene that produces factor viii-generate cdna of the factor viii gene using reverse transcriptase- transform the vector into e. coli bacterium-the factor viii protein is isoloated from human tissues-insert the factor viii cdna into a bacterial vector near a promoter site-human dna is introduced into the bacterial cell using direct injection by a small needle-isolate the mrna of the factor viii genee. coli expresses factor viii

What part of the brain is highlighted in the diagram below?

1. seamount a raised footwall block between normal fault creates this 2. syncline break between rocks where a hanging wall rises relative to a footwall 3. hot spring on rolling hills, this a dip between hills 4. volcanic neck created when a block with hanging walls slips down between normal faults 5. caldera underwater volcano that never reaches above sea level 6. horst natural hot water on earth's surface containing many minerals 7. graben underwater volcano whose top is eroded flat by waves 8. crater less than a mile in diameter; looks like a bowl at the top of a volcano 9. guyot magma that filled the central vent that remains after the volcano has eroded 10. reverse fault over 1 mile in diameter; looks like a bowl over a volcano

Resperation is a way to release carbon back into the atmosphere