You want to use a plasmid to transform an e. coli cell with a cry toxin gene from bacillus thuringiensis in order to express the toxin in e. coli. in addition to the gene of interest, what other elements must be included on the plasmid? select all that apply. a gene encoding an e. coli-specific dna polymerase. an origin of replication. a selectable marker (e. g., a gene encoding antibiotic resistance). a promoter allowing the expression of the cry gene in an e. coli host.

1) An origin of replication.

2) A selectable marker (e.g., a gene encoding antibiotic resistance).

Others are not necessary.

Explanation: In genetic engineering, three (3) things are responsible for manipulating nucleic acids, they are: plasmid, an origin of replication, and a selectable marker.

there are 35 white marbles

step-by-step explanation:

y- yellow marbles

4y + 5 = 45

subtract 5 from both sides

4y = 40

divide for from both sides

y = 10

plug in 10 to get 40 yellow marbles then subtract by 5 to get 35 marbles

thus there are only 10 yellow marbles