Phosphatidylcholine and sphingomyelin both contain two long hydrocarbon chains, a phosphate, and the alcohol choline as a polar group. which of the following methods could you use to distinguish between these two phospholipids? □ hydrolysis with mild base separation by chromatography on silica gel acid hydrolysis followed by reaction with cupric (cu ) ion acid hydrolysis followed by reaction with sanger reagent (1-fluoro-2,4-dinitrobenzene)