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Biology, 06.12.2019 17:31 Lovebamagirl12
You are interested in using crispr to edit a particular gene in the genome. select the correct order of events that must occur to introduce a double stranded dna break using crispr/cas9.
1. small guide rna (sgrna) hybridizes with the target dna
2. cas9 cuts dna
3. cas9/sgrna complex binds pam sequence
4. sgrna binds cas9
5. cas9 unzips dna
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Answers: 3
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