Astudent receives the sequencing results for a bacterial enome. after analyzing the results, he is disappointed to ind a large gap in the alignment of his contigs. the student decides to obtain the missing sequence data via polymerase chain reaction (pcr). gap select the correct requirements for using pcr to determine the sequence of this gap. one primer must be complementary to the 3-beginning contig at the gap. contig ends must lie close enough together for the reliable amplification of the entire length of the gap. one primer of the pair must not contain repeated genome sequences. at least one primer must be complementary to the 5-beginning contig at the gap. ? gaps must be shorter than 200 bp in order to amplify accurately.

not always but somethings can be reproduced in a laboratory.

answer australia and antarctica;

1. the geologists have established a set of principals that can can be used o determine the relative ages of rock. this principal is applied to sedimentary and volcanic rocks. the rocks exposed to wall of grand canyon has layers and by studying the number of horizontal layers in the rock(strata) the relative age can be determined. the layer first formed will appear at the bottom and the the layer of strata last formed appear at the top. by using this information the approximate age of rocks can be determined.

2. carbon dating is used to find the relative age of rock in numbers which means for the rocks that has age between 1-70,000 years.

3. for the rocks of age range between 1,000 - billion of years potassium-argon dating is used.