In order to visualize if a bacteria grown on a starch agar plate is positive for amylase, ( must be added to the surface of the agar. if the bacteria is indeed positive for this enzyme, one would observe (2) around the bacterial growth.
(1) crystal violet / (2) an area of blue-purple staining
(1) gram iodine / (2) an area of blue- purple staining
(1) gram iodine / (2) an area of clearing or a non-stained halo
(1) methylene blue / (2) an area of clearing or a non-stained hal

Answer

1) Gram Iodine 2) area of clearing or non stained halo

Explanations:

Amylase catalyzes the breakdown of starch into maltose, a disaccharide ( two joined monosaccharide like glucose). This action was the reason why there was zone of clearing since starch molecules has been broken down. If present starch give a blue black colouration with Iodine.

image result for how many calories of energy will 20 grams of carbohydrates provide in one gram provides 4 calories

consuming the right balance of protein, carbohydrates and fat can you maintain a healthy weight and optimize your energy levels. protein and carbohydrates both contain 4 calories per gram, while fat provides 9 calories per gram.